cellspace.bio

Cellspace is an innovation project from the Hybrid Technology Hub at the University of Oslo and Oslo University Hospital.

Cellspace is an in Innovation project from the Hybrid Technology Hub at the University of Oslo and Oslo University Hospital

TECHNOLOGY

CellSpace provides a scalable next-generation micro-physiological system (MPS platform for disease modeling and drug testing.

Our revolving organ-on-chip (rOoC) technology is based on a patented fluidic layout combined with a 3D tilting apparatus that produces a directed gravity-driven flow without the need for pumps and tubes.

The rOoC platform enables the functional culture of multiple 3D organ models, endothelial cells, and the integration of circulating cells such as immune cells or particles. All these elements can be coupled together in different combinations in one microfluidic device, allowing the development of complex tissue and organ representations and advanced disease models.

Our pump-less approach ensures high scalability and user-friendliness for drug discovery and drug testing in both academic and industrial R&D laboratories.

MPS platform

MICROFLUIDICS

Pump-less revolving organ on chip ( OoC ) platform that addresses central shortfalls of the OoC field in a robust and scalable configuration

Supports multiple 3D organ models, blood vessels and circulating cells (e.g. immune cells)
Generates directional flow without pumps
Plate and single chip format
Designs optimized for different organ models
Compatible with automated pipetting robots
No pumps needed
High scalability
User-friendly
Life-cell imaging
PDMS-free (no drugs absorption)

Single-use rOoC chips are available in different layouts, optimized for different organ models and biological questions. rOoC chips are shipped in a sterile, ready-to-use bag as single chips or as array of several rOoCs in the well plate format. We currently offer following rOoC layouts:

Single-Loop	Loop-in-Loop	Infinity-Loop
Combination of two large organoid/cell chamber in one perfusion loop	Combination of two smaller organoid chambers placed between two independent perfusion loops	Incorporates a membrane between two separate perfusion loops. Each loop include one organoid chamber.
Suitable for demanding or big organoids, vascularization and immune infiltration	Suitable for applications that require gradient formation, for studying immune infiltration and vascularization and organ interaction	This unique layout adds an endothelial/epithelial barrier between both loops thus adding the option to study drug or immune cell transport/adsorption.

Single-Loop

Combination of two large organoid/cell chamber in one perfusion loop
Suitable for demanding or big organoids, vascularization and immune infiltration

Loop-in-Loop

Combination of two smaller organoid chambers placed between two independent perfusion loops
Suitable for applications that require gradient formation, for studying immune infiltration and vascularization and organ interaction

Infinity-Loop

Incorporates a membrane between two separate perfusion loops. Each loop include one organoid chamber.
This unique layout adds an endothelial/epithelial barrier between both loops thus adding the option to study drug or immune cell transport/adsorption.

We also provide a customized tilting system to run the devices. Please contact us for more information and pricing.

MPS assays

METABOLISM ON CHIP

Human model of pancreas liver interaction for Type II diabetes, NAFLD (non alcohol fatty liver disease ) and Obesity studies

Long-term functionality and viability of organoids
Functional cross-talk between
pancreas and liver models
Reproducible disease phenotype
Suitable for drug testing
Possibility to incorporate vascular
and immune components
PDMS-free (no drugs absorption
IP: National phase in December 2023

MPS Assay

IMMUNE CHIP

Physiologically relevant conditions for Studies of circulating immune cells their interaction with 3D organoid models and blood vessels

Stable viability
No activation
No trapping
Migration following a gradient of chemokines
Interaction with organoids
Extravasation
No pumps needed
High scalability
User-friendly
Life-cell imaging
PDMS-free (no drugs absorption
IP: National phase in December 2023

MPS Assay

CANCER ON CHIP

Novel platform for an advanced human model of metastasis cascade

Co-culture of 3D primary Tumor and liver organoids (secondary site)
Vascularization model
Membrane-free endothelial barrier for
intravasation /extravasation
NK-mediated killing assay
Possibility to incorporate immune components

MPS platform

MICROFLUIDICS

Pump-less revolving organ on chip ( OoC ) platform that addresses central shortfalls of the OoC field in a robust and scalable configuration

Supports multiple 3D organ models, blood vessels and circulating cells (e.g. immune cells)
Generates directional flow without pumps
Plate and single chip format
Designs optimized for different organ models
Compatible with automated pipetting robots
No pumps needed
High scalability
User-friendly
Life-cell imaging
PDMS-free (no drugs absorption)

Single-Loop	Loop-in-Loop	Infinity-Loop
Combination of two large organoid/cell chamber in one perfusion loop	Combination of two smaller organoid chambers placed between two independent perfusion loops	Incorporates a membrane between two separate perfusion loops. Each loop include one organoid chamber.
Suitable for demanding or big organoids, vascularization and immune infiltration	Suitable for applications that require gradient formation, for studying immune infiltration and vascularization and organ interaction	This unique layout adds an endothelial/epithelial barrier between both loops thus adding the option to study drug or immune cell transport/adsorption.

We also provide a customized tilting system to run the devices. Please contact us for more information and pricing.

MPS assays

METABOLISM ON CHIP

Human model of pancreas liver interaction for Type II diabetes, NAFLD (non alcohol fatty liver disease ) and Obesity studies

Long-term functionality and viability of organoids
Functional cross-talk between
pancreas and liver models
Reproducible disease phenotype
Suitable for drug testing
Possibility to incorporate vascular
and immune components

MPS Assay

IMMUNE CHIP

Physiologically relevant conditions for Studies of circulating immune cells their interaction with 3D organoid models and blood vessels

Stable viability
No activation
No trapping
Migration following a gradient of chemokines
Interaction with organoids
Extravasation
No pumps needed
High scalability
User-friendly
Life-cell imaging
PDMS-free (no drugs absorption
IP: National phase in December 2023

MPS Assay

CANCER ON CHIP

Novel platform for an advanced human model of metastasis cascade

Co-culture of 3D primary Tumor and liver organoids (secondary site)
Vascularization model
Membrane-free endothelial barrier for
intravasation /extravasation
NK-mediated killing assay
Possibility to incorporate immune components

TEAM

CellSpace is an innovation project from Oslo, Norway that aims to spin out as a startup company from the Hybrid Technology Hub – Centre of Excellence (HTH-CoE) at the University of Oslo.

The technology has been developed by Mathias Busek (Ph.D.), who has more than 10 years of experience in the OoC field and pioneered an OoC platform with integrated pneumatic micro-pumps at TU Berlin (now used for drug screening by TissUse GmbH). He is leading the platform and technological developments at CellSpace. Biological use-cases development and incorporation of advanced 3D culture models into the platform is led by Alexandra Aizenshtadt (Ph.D.), who has both entrepreneur and academic experience (more than 10 years of experience in cell biology and disease modeling, and worked in interdisciplinary commercial and academic teams). The team is complemented by Prof. Stefan Krauss, director of the HTH-CoE and an international expert in developmental biology and drug development with a broad experience in academic/industrial collaborations.